Acyrthosiphon pisum from susceptible strains alfalfa - SRA

SRX18238383: Acyrthosiphon pisum from susceptible strains alfalfa
1 OXFORD_NANOPORE (MinION) run: 6.7M spots, 6.1G bases, 5.2Gb downloads

Design: 1ug total RNA was prepared for cDNA libraries using cDNA-PCR Sequencing Kit (SQK-PCS109) protocol provided by Oxford Nanopore TechnologiesONT. Briefly,the template switching activity of reverse transcriptases enrich for full-length cDNAs and add defined PCR adapters directly to both ends of the first-strand cDNA. And following cDNA PCR for 14 circles with LongAmp Tag (NEB). The PCR products were then subjected to ONT adaptor ligation using T4 DNA ligase (NEB). Agencourt XP beads was used for DNA purification according to ONT protocol. The final cDNA libraries were added to FLO-MIN109 flowcells and run on PromethION platform at Biomarker Technology Company (Beijing, China).

Submitted by: Gansu Agricultural University

Study: Transcriptomic and functional analyses of the adaptation mechanism of pea aphid to alfalfa

PRJNA892899 • SRP407454 • All experiments • All runs

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In order to understand the adaptability of pea aphids to different alfalfa varieties. We analyzed the differentially expressed genes of pea aphid in different alfalfa varieties by using RNA-Seq.

Sample: Acyrthosiphon pisum feed on resistant strains of Medicago sativa called Gannong 5

SAMN31399377 • SRS15734385 • All experiments • All runs

Organism: Acyrthosiphon pisum

Library:

Name: GF3

Instrument: MinION

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Oligo-dT

Layout: SINGLE

Runs: 1 run, 6.7M spots, 6.1G bases, 5.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR22261921	6,660,420	6.1G	5.2Gb	2022-11-11

ID:: 25221859

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